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Шакир Ирина Васильевна

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Профессор кафедры биотехнологии (Кафедра биотехнологии)

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+7 (495) 495-23-79

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shakir.i.v@muctr.ru

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Уровень образования Высшее
Квалификация

Инженер-технолог

Преподаваемые учебные предметы, курсы, дисциплины (модули)

Биотехнология биополимеров

Пищевая биотехнология

Технология ферментных препаратов

Практикум по общей микробиологии

Учёная степень

Кандидат технических наук

Учёное звание Доцент
Наименование направления подготовки и (или) специальности

Технология микробиологических производств

Данные о повышении квалификации и (или) профессиональной переподготовке

Удостоверение о повышении квалификации № 772401673329 от 15.04.2016, рег.номер 6763 "Сельскохозяйственная микробиология. Биологические средства защиты растений"", 72 часа, ФГБОУ ВО ""РХТУ им. Д.И. Менделеева"

Удостоверение о повышении квалификации № 771801453041 от 25.05.2018, рег. номер 7419 "Информационные технологии в дистанционном, сетевом и смешанном обучении"", 16 часов, ФГБОУ ВО ""РХТУ им. Д.И. Менделеева"

Общий стаж работы 34 года (с 01.12.1989)
Стаж работы по специальности 34 года (с 01.12.1989)

 Разработка малоотходных и безотходных биотехнологических процессов получения биомассы микроорганизмов, продуктов микробиологической переработки, первичных метаболитов на различных органических субстратах, в том числе отходах сельского хозяйства, пищевой, молокоперерабатывающей, пивоваренной, спиртовой и других отраслей промышленности.

Публикации

Prospects for the use of cereal hydrolysates fermented with lactobacilli to produce new probiotic beverages / V. I. Panfilov, B. A. Karetkin, E. A. Kalugina et al. // 19th International Multidisciplinary Scientific GeoConference SGEM 2019. — Vol. 19 of Advances in Biotechnology. — Alexander Malinov Sofia, Bulgaria, 2019. — P. 867–874. New functional foods produced by fermentation of pretreated with amylolytic and proteolytic enzymes cereal raw materials suspensions by probiotics are presented. Whole meal wheat flour comprising organic compounds and microelements was used as source of nutrients. Duozyme (Novozymes) amylases (1% by weight of the substrate) and Protex 40E (Genencor) proteases (2% of the protein content in flour) were applied for hydrolysis. The starter culture was one of the gram-positive probiotic lactic acid bacteria Lactobacillus paracasei (VKPM B-4079), L. rhamnosus (VKPM B-8238), L. plantarum (VKPM B-7583), L. bulgaricus Lb 14 (VKPM B-4626) or L. acidophilus (VKPM B-2105). The count of lactic acid bacteria viable cells was between 8 and 9 log CFU/mL after 12 h of fermentation. The concentration of lactic acid in the fermented product considered as factor of antagonistic activity against pathogenic bacteria was from 5.5 to 10.2 mg/mL. The highest growth (9.00 ± 0.30 log CFU/mL), as well as the highest final concentration of lactic acid (10.2 mg/mL) were observed for L. rhamnosus culture. The final pH values were in the range 3.37-3.68. The count of lactobacilli was not less 7-8 log CFU/mL after the 28 days of refrigerator storage (4 C). Quantitative assessment of fermented beverages organoleptic properties were carried out on 27 volunteers (female and male aged 22 to 60 years). The highest intensity of aroma was observed in the samples fermented with L. rhamnosus and L. paracasei, the samples fermented with L. bulgaricus were the sweetest one. The most acidic samples were obtained with L. plantarum. Interestingly, the products fermented by L. acidophilus, L. plantarum, L. bulgaricus, had similarities in such values as odor, total intensity of aroma, bread aroma, sweetness, and the samples with L. rhamnosus and L. paracasei combined indicators: color, smell, total intensity of aroma, bread aroma, sweetness and bitterness. So cereals enzymatic hydrolysates can compete with dairy products due to the fool chemical composition. The fermented cereal beverages recognized as functional foods in present study have great potential for implementation. [ DOI ]

Sunflower protein enzymatic hydrolysis and plant peptone production as a source of nitrogen for nutrient media / D. V. Baurin, J. M. Epishkina, A. V. Baurina et al. // 19th International Multidisciplinary Scientific GeoConference SGEM 2019. — Vol. 19 of Advances in biotechnology. — Albena, Bulgaria, 2019. — P. 939–946. Industrially defatted non-dehulled sunflower meal (SFM) with a protein content 22%, fibre 38% and cellulose 23% was separated into several fractions: protein fraction and lignocellulosic fraction, then they can be used for bioconversion into value added products. The protein concentrate (PC) contained 83% of protein. Hydrolysis of this concentrate by the commercial proteases, such as Proteinase T, Protex 7L, Protex 40E (Danisco Genecor, Denmark) and Pancreatin (Biosynthesis, the Russian Federation) can transform this concentrate into protein hydrolysate with higher content of amine nitrogen. Amino nitrogen content for different enzymatic preparations varied from 1.5 to 2.8 mg per ml and was significantly higher when combination of preparations was used. Composition and high solubility of sunflower hydrolysates at wide range of pH values proved that the product had potential for sunflower peptone production and its use as a nitrogen source for nutrient media. Crude protein was determined by the Kjeldahl method. Amine nitrogen was measured by Sorensen method (formaline titration method). Several bacterial strains were chosen as test objects and they proved to accumulate a significant amount of biomass with high cell count per ml. [ DOI ]

Wastewater treatment of single сell protein production with b.subtilis / A. V. Baurina, D. V. Baurin, J. M. Epishkina и др. // 19th International Multidisciplinary Scientific GeoConference SGEM 2019. — Т. 19 из Advances in biotechnology. — Albena, Bulgaria, 2019. — С. 1037–1044. This paper compares four growth models (Logistic Model, Gompertz Model, Richards Model and Stannard Model) using the experimental data of wastewater treatment from a single sell protein production with Bacillus subtilis in order to describe bacterial biomass growth. One model was used to describe carbohydrate consumption during the bacterial fermentation. This study was carried at the Department of Biotechnology (Mendeleev University of Chemical Technology of Russia) as part of deproteinized sunflower meal bioconversion into a highly added value product research. The temporal profiles of carbohydrates unmetabolized during single cell protein production with the yeasts, and CFU/mL were modelled with a set of ordinary differential equations solved using MATLAB software. The models were validated by the experimental data based on goodness of fit criteria; R2, MSE, and SD values. The coherence between the models and experimental results demonstrated that the models were reasonable for the prediction of the fermentation process dynamic behavior. The models gave more information about the operation conditions of the fermentation and they could be applied for further development and scaling of wastewater treatment during the single cell protein production. [ DOI ]

Fungi cellulases for crude fibre reduction in plant raw materials / J. M. Epishkina, V. I. Panfilov, D. V. Baurin et al. // International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management. — Vol. 18 of Advances in biotechnology. — Albena, Bulgaria, 2018. — P. 207–213. [ DOI ]

Mathematical modeling of s. cerevisiae growth on deproteinized plant raw material hydrolysates / A. V. Tur, V. I. Panfilov, D. V. Baurin et al. // International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management. — Vol. 18 of Advances in biotechnology. — Albena, Bulgaria, 2018. — P. 353–360. [ DOI ]

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