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Преподаватели и сотрудники

Белов Алексей Алексеевич

Белов Алексей Алексеевич

Занимаемые должности

Профессор (Кафедра биотехнологии)





Сайт https://muctr.ru
Уровень образования Высшее


Преподаваемые дисциплины

Методы энзимологии

Современные проблемы медицинской и фармацевтической биотехнологии

Учёная степень

Доктор технических наук

Наименование направления подготовки и (или) специальности

Технология неорганических веществ и химических удобрений

Общий стаж работы 11 лет (с 01.09.2007)
Стаж работы по специальности 11 лет (с 01.09.2007)


Formulation and production of a novel pharmaceutical substance for treatment of infected wounds - a chitosan chymopsin complex / A. A. Belov, L. L. Brkich, G. E. Brkich et al. // Journal of Pharmaceutical Sciences and Research. — 2018. — Vol. 10, no. №6. — P. 1310–1313. Abstract Wound infection represent a serious threat to wounded patients all over the world. Untreated wounds may result in bacterial wound infections, such as gas gangrene and tetanus. These in turn may lead to long term disabilities, chronic wound or bone infection, and, ultimately, death. Appropriate management of infected wounds is important to reduce the likelihood of life-threatening complications. Chitosan chymopsin complex (CCC) is a novel pharmaceutical substance consisting of chymopsin (a combination of two proteolytic enzymes: trypsin and chymotrypsin) and acid-soluble chitosan (a natural high molecular weight polysaccharide). This substance, either alone or together with antiseptic and/or anesthetic agents, can be used in treatment of infected wounds of different etiology. Synthesis, and production of active pharmaceutical ingredients (API) using biotechnological methods are the most demanding and costly stages of drug manufacturing. Therefore, a thoughtfully performed formulation can significantly reduce production costs, make manufacturing less labour-intensive, and, of course, improve API performance and quality. The paper describes the process of formulation of a novel API which possesses complex therapeutical activity, allows for faster wound healing and effective clearance of wound surface from pus and necrotic debris. Keywords: active pharmaceutical ingredient, chitin deacetylation, chitosan chymopsin complex (ССС), crab hepatopancreas, infected wounds treatment, polyelectrolyte complex (PEC), proteolytic complex (PC), proteolytic unit (PU).

Halobacterium salinarum storage and rehydration after spray drying and optimization of the processes for preservation of carotenoids / S. V. Kalenov, M. G. Gordienko, E. D. Murzina et al. // Extremophiles. — 2018. — Vol. 22, no. 3. — P. 511–523. [ DOI ]

Miramistin as an antimicrobial component in the innovative substance of chitosan-miramistin complex (cmc) for the treatment of infected wounds of various genesis / L. L. Brkich, N. V. Pyatigorskaya, G. E. Brkich et al. // Journal of Pharmaceutical Sciences and Research. — 2018. — Vol. 10, no. 8. — P. 2027–2029.

Elina E. D., Elizaveta E. S., Alexey A. B. The effect of immobilization, drying and storage on the activity of proteinases immobilized on modified cellulose and chitosan // Process Biochemistry. — 2018. — Vol. 64, no. January. — P. 213–220. Nowadays the wound healing dressings based on modified cellulose, chitosan and various immobilized therapeutic agents are commonly used. This study disclosed the preparation of dialdehyde cellulose (DAC), immobilization of chitosan and different enzymes (trypsin, bromelain, and proteolytic complex from hepatopancreas of crab) and demonstrated the analysis of enzymatic activity of obtained materials during immobilization, drying and storage. The research also covered a possible structural scheme of obtained material and the methods of its production. The exponential equations were presented as a model, developed as a mathematical description of enzymatic activity loss, which was studied during the stated processes. The optimal conditions for the product with the preservation of enzymatic activity were chosen on the basis of developed equations. It was stated that chitosan stabilizes the analyzed proteinases during drying and storage: for proteolytic complex from hepatopancreas of crab (PC) over 150%, and for bromelain − 600%. The main destructive factor for the obtained enzymatic preparation during storage is water (in immobilized products). The humidity of the used materials was 5%, therefore 50 mg of water per 1 mg of immobilized protein was reported to be the critical concentration that caused the loss of enzymatic activity of the studied enzymes. [ DOI ]

Влияние высушивания и сроков хранения на биологические свойства хитозановых композитов содержащих ферменты и различные терапевтические агенты / Э. Э. Досадина, Е. Е. Савельева, Л. Л. Бркич и др. // Бутлеровские сообщения. — 2018. — Т. 55, № 7. — С. 64–73.